Crimean-Congo Hemorrhagic Fever Virus Gn Bioinformatic Analysis and Construction of a Recombinant Bacmid in Order to Express Gn by Baculovirus Expression System
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منابع مشابه
Crimean-Congo Hemorrhagic Fever Virus Gn Bioinformatic Analysis and Construction of a Recombinant Bacmid in Order to Express Gn by Baculovirus Expression System.
BACKGROUND Crimean-Congo hemorrhagic fever virus (CCHFV) is a member of the nairovirus, a genus in the Bunyaviridae family, which causes a life threatening disease in human. Currently, there is no vaccine against CCHFV and detailed structural analysis of CCHFV proteins remains undefined. The CCHFV M RNA segment encodes two viral surface glycoproteins known as Gn and Gc. Viral glycoproteins can ...
متن کاملcrimean-congo hemorrhagic fever virus gn bioinformatic analysis and construction of a recombinant bacmid in order to express gn by baculovirus expression system
results primary, secondary, and 3d structure of cchfv gn were obtained and pcr reaction with m13 forward and reverse primers confirmed the generation of recombinant bacmid dna harboring gn coding region under polyhedron promoter. conclusions characterization of the detailed structure of cchfv gn by bioinformatics software provides the basis for development of new experiments and construction of...
متن کاملStructural characterization of the Crimean-Congo hemorrhagic fever virus Gn tail provides insight into virus assembly.
The RNA virus that causes the Crimean Congo Hemorrhagic Fever (CCHF) is a tick-borne pathogen of the Nairovirus genus, family Bunyaviridae. Unlike many zoonotic viruses that are only passed between animals and humans, the CCHF virus can also be transmitted from human to human with an overall mortality rate approaching 30%. Currently, there are no atomic structures for any CCHF virus proteins or...
متن کاملPhylogeography of Crimean Congo Hemorrhagic Fever Virus
Crimean Congo hemorrhagic fever virus (CCHFV) is one of the most severe viral zoonozes. It is prevalent throughout Africa, Asia and southern Europe. Limited availability of sequence data has hindered phylogeographic studies. The complete genomic sequence of all three segments of 14 Crimean Congo hemorrhagic fever virus strains isolated from 1958-2000 in Russia, Central Asia and Africa was ident...
متن کاملEmergence of a New Genotype of Crimean-Congo Hemorrhagic Fever Virus in Iran
Background and Aims: Crimean-Congo hemorrhagic fever (CCHF) is a fatal viral disease that occurs in approximately 30 countries. It has the most extensive geographic range among the tick-borne viruses that affect human health. CCHF viruses have a tripartite RNA genome consisting of large (L), medium (M) and small (S) segments. This study was undertaken to determine the genetic relationship of CC...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2015
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.25502